Journal: International Journal of Biological Sciences

Article Title: Epigenetic Down-Regulation of Sirt 1 via DNA Methylation and Oxidative Stress Signaling Contributes to the Gestational Diabetes Mellitus-Induced Fetal Programming of Heart Ischemia-Sensitive Phenotype in Late Life

doi: 10.7150/ijbs.33044

Figure Lengend Snippet: Effect of GDM on the protein expression of Sirt1 and p-Akt/Akt in male offspring. The hearts were isolated from both control (□) and STZ-treated (■) groups of male offspring. The protein abundance in the LV tissue was determined by Western blot analysis. The Sirt1 protein density ( A ) and P-Akt/Akt protein density ( B ) in the LV tissues of the groups which had not treated with NAC. The Sirt1 protein density ( C ) and P-Akt/Akt protein density ( D ) in the LV tissues of the groups which had treated with NAC. Data are means ± SEM (n=4 animals/group). *P < 0.05 vs. control, as determined by Student's t-test.

Article Snippet: The membranes were incubated at 4 °C with primary antibodies against Sirtuin 1 (SIRT1) (Cell Signaling Technology, USA), DNA methyltransferase (DNMT) 1, 3a, 3b (Cell Signaling Technology, USA), NADPH oxidase (NOX) 1, 2, 4 (Boster Biological Technology, USA), p-Akt (Cell Signaling Technology, USA), Akt (Cell Signaling Technology, USA), Beclin1 (Cell Signaling Technology, USA), Atg5 (Cell Signaling Technology, USA), LC3B (Cell Signaling Technology, USA) and GAPDH (MilliporeSigma, USA), respectively.

Techniques: Expressing, Isolation, Control, Quantitative Proteomics, Western Blot